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The kit is based on a research cooperation with the University of Konstanz. This cooperation developed a new rapid Covid-19 detection process accelerating SARS-CoV-2 detection by skipping RNA extraction (see News below). This process, is based on an innovative DNA polymerase, developed and produced by myPOLS Biotec.
myPOLS Biotec has now decided to offer the new process in a Direct SARS-CoV-2 RT-PCR detection kit w/o RNA extraction. The kit contains an in-house developed master mix and a duplex primer/probe mix for both SARS-CoV-2 (N1 gene specific) and RNaseP control. It´s for research use only.
News published November 23, 2020
The study in collaboration with the local University of Konstanz, research group of Prof. Hauck, has now been published in PLOS ONE: Detection of SARS-CoV-2 from raw patient samples by coupled high temperature reverse transcription and amplification
News published November 03, 2020
We almost reach the end of 2020. Maybe you already plan for 2021 and want to fill up your stock? Use our special End-of-Year Offer: get 15% discount for our master mixes and polymerases! We deliver in 2020 or 2021, depending on your needs.
Have you already seen our newest product?
Running RT-PCR tests?
Use our Volcano3G® RT-PCR Probe 2x Master Mix: A unique master mix with an engineered, truly thermostable Taq DNA polymerases with reverse transcriptase activity, fast start functionality due to a hotstart aptamer formulation and an already included lysis function for direct RT-PCRs (suitable for SARS-CoV-2/Covid-19 detections). All this for an attractive price. Enter the product page!
*This offer is limited to off-the-shelf products ordered with the special order code. Services, bulk amounts and customizations are excluded. myPOLS Biotec GmbH reserves the right to end this promotion at any time. Offers cannot be combined with other discounts or promotions. Expires November 30, 2020.
News published October 14, 2020
A study in collaboration with the local University of Konstanz, research group of Prof. Hauck, has been published and can now be found on the medRxiv pre-print server: https://www.medrxiv.org/content/10.1101/2020.05.19.20103150v1
As shown in the study, Volcano3G® master mix can be used to reliably detect SARS-CoV2 in extracted samples using the CDC-approved N1 and RNaseP qPCR-assay. When using an additional, high-melting reverse transcription primer, Volcano3G master mix even displayed lower Ct values than other commercially available RT-PCR mixes.
Since RNA extraction is time consuming and the availability of the extraction kits is scarce due to the world wide demand, we additionally explored to detect SARS-CoV2 directly from raw, dry oral swabs. These swabs are simply rinsed with RNase-free water and the sample is then directly added to the RT-PCR reaction. With this setup, samples of patients with a medium to high viral load were accurately detected in agreement with the results of extracted RNA samples. Due to inhibitory effect of the raw sample on the reaction, however, the input of the diluted sample is too low to achieve a reliable detection of SARS-CoV2 in patients with mild symptoms (and hence low viral titers).
We are currently exploring ways to improve the sensitivity of this approach and to launch it as an official, approved diagnostic kit as soon as possible.
myPOLS is upscaling Volcano3G® productions significantly in order to supporting the fight against Corona virus.
We are proud to announce that HiDi DNA polymerase and HiDi Taq DNA polymerase are becoming gold-standard enzymes for mutation detections as recent publications in high-impact journals demonstrate. Please see below
H. Matsunari et al., describing genome edited clones and allele-specific PCR with HiDi DNA polymerase - Link to the scientific publication
M. Serif et al., describing TALEN-mediated gene knockouts and identification by HiDi DNA polymerase - Link to the scientific publication
T. Sakurai et al., describing a CRISP-Cas9 induced mutation identification method - Link to the scientific publication
Ramon (our CEO) was giving a talk at the qPCR conference in Freising this spring. His talk was recorded and is now online available - have a look! (passwort protected video stream (psw is: GQ2019)
After months of tedious work we are proud to announce that our Quality Management system has been certified according to the ISO-Norm 13485:2016 (more details can be found here)
We have successfully established a Direct from Blood Genotyping PCR mix!
Our "DirectBlood Genotyping PCR Mix" allows real-time PCR based genotyping without any previous DNA isolation, directly from blood samples!
Currently we are further developing specific SNP typing assays. They will be launched under research-use only as soon as possible. If you are interested, please provide us with the rs-number of the SNP your are interested in, to receive an individual quotation.