Publications with myPOLS Biotec products: (Examples)

Our products have been successfully used in various applications worldwide:
(Click on the link to go to the specific references)

1. DirectBlood Genotyping PCR Kit

2. Volcano Product Family

3. qPCR Probe 2x LyoCake Master mix

4. HiDi DNA Polymerase Product Family

Download the publication list here (Last update: 12.01.2021)

1.    DirectBlood Genotyping PCR Kit

Real-time PCR based HLA-B*27 screening directly in whole blood.
"…Therefore, in order to refine HLA‐B*27 analysis the aim of our study was to develop a valid real‐time TaqMan PCR based protocol to detect the HLA‐B*27 allele directly in blood samples. The protocol combines a commercially available PCR master mix (myPOLS Biotec, Konstanz, Germany) including a mutant Taq polymerase for direct blood PCR together with real‐time TaqMan PCR‐assays for amplification of B*27 specific regions in exon 2 and exon 3 of the HLA…"
Geiger K, Zach C, Leiherer A, Fraunberger P, Drexel H, Muendlein A.
HLA. 2020 Mar;95(3):189-195.
Link to publication: https://doi.org/10.1111/tan.13767

Real-time PCR based detection of the lactase non-persistence associated genetic variant LCT-13910C>T directly from whole blood.
"…The protocol combines the commercially available DirectBlood Genotyping PCR Kit (myPOLS Biotec) together with an in-house developed TaqMan-assay for real-time detection of the LCT-13910C>T variant. The DirectBlood Genotyping PCR Kit contains an inhibition-resistant mutant of Thermus aquaticus (Taq) DNA polymerase and potentially non-disclosed substances to increase PCR inhibitor resistance…"
Muendlein A, Leiherer A, Zach C, Brandtner EM, Fraunberger P, Drexel H, Geiger K.
Mol Biol Rep. 2019 Apr;46(2):2379-2385
Link to publication: https://doi.org/10.1007/s11033-019-04696-9

Direct blood PCR: TaqMan-probe based detection of the venous thromboembolism associated mutations factor V Leiden and prothrombin c.20210G>A without DNA extraction.
"… F5 c.1691G>A (p.R506Q) and F2 c.20210G>A mutations were determined in 205 EDTA anti-coagulated whole blood samples from patients who underwent routine clinical genotyping using the DirectBlood Genotyping PCR Kit (myPOLS Biotec, Konstanz, Germany) together with in-house developed TaqMan primer-probe assays…"
Geiger K, Leiherer A, Brandtner EM, Fraunberger P, Drexel H, Muendlein A.
Clin Chim Acta. 2019 Jan;488:221-225
Link to publication: https://doi.org/10.1016/j.cca.2018.11.016

2.    Volcano Product Family

"...RNA (5 μl) was added to each RT-PCR mixture (Vulcano3G; myPOLS), and RNase P was detected by TaqMan (CDC-recommended primers/probe).
..."
Zander J, Scholtes S, Ottinger M, Kremer M, Kharazi A, Stadler V, Bickmann J, Zeleny C, Kuiper JWP. , and Hauck CR
ASM Journals Microbiology Spectrum Vol. 9, No. 1 14 July 2021
Link to publication: https://doi.org/10.1128/Spectrum.00361-21

Detection of SARS-CoV-2 from raw patient samples by coupled high temperature reverse transcription and amplification.
"…Throughout this study high-temperature RT-PCR on RNA purified from either the in vitro transcribed viral genome fragment or the swab material was performed with the Volcano3G RT-PCR Probe 2x Master Mix (in short: Volcano3G) (myPOLS Biotec, Konstanz, Germany) using the CDC-approved N1 primer/probe mix from Integrated DNA Technologies (IDT, San Diego, CA, USA)…"
Kuiper JWP, Baade T, Kremer M, Kranaster R, Irmisch L, Schuchmann M, Zander J, Marx A, Hauck CR.
PLoS One. 2020 Nov 2;15(11):e0241740.
Link to publication: https://doi.org/10.1371/journal.pone.0241740

Pharmacological LRH-1/Nr5a2 inhibition limits pro-inflammatory cytokine production in macrophages and associated experimental hepatitis.
"…Murine Nr5a2 and corresponding Actb transcripts were detected using a FAM-based TaqMan probe and the Volcano3G RT-PCR Probe Master Mix (myPols Biotec GmbH)…"
Schwaderer J, Phan TS, Glöckner A, Delp J, Leist M, Brunner T, Delgado ME.
Cell Death Dis. 2020 Feb 28;11(2):154.
Link to publication: https://doi.org/10.1038/s41419-020-2348-9

Reverse-transcription quantitative PCR directly from cells without RNA extraction and without isothermal reverse-transcription: a 'zero-step' RT-qPCR protocol.
"…For a ‘zero-step’ RT-qPCR, cells were lyzed directly in the culture well using VolcanoCell2G Lysis Buffer (myPOLS Biotec) for 15 min at 4 °C. Cell lysate dilutions were prepared in the lysis buffer and stored at −80 °C. Thawed diluted supernatant from approximately 1500 cells was then used as a template for the RT-qPCR reaction. Reaction mixtures (10 µl) contained 5 µl of VolcanoCell2G 2× RT-PCR Master Mix (myPOLS Biotec), 0.1 µM of the respective hydrolysis probe, and 0.4 µM of the respective forward and reverse primers…"
Chovancova P, Merk V, Marx A, Leist M, Kranaster R.
Biol Methods Protoc. 2017 Jan;2(1):bpx008.
Link to publication: https://doi.org/10.1093/biomethods/bpx008

3.    qPCR Probe 2x LyoCake Master mix

SPLICELECT™: an adaptable cell surface display technology based on alternative splicing allowing the qualitative and quantitative prediction of secreted product at a single-cell level.
“…The following probes and primers were used: 111_spl22.3_short_PROBE1, 111_spl22.3_short_FW3 and 111_spl22.3_short_REV2 for the short splice transcript and 111_spl22.6_long_PROBE1, 111_spl22.3_short_FW3 and 111_spl22.3_short_REV2 for the long transcript. The qPCR Probe 2x LyoCake Master mix (MyPols) was used…”
Aebischer-Gumy C, Moretti P, Ollier R, Ries Fecourt C, Rousseau F, Bertschinger M.
MAbs. 2020 Jan-Dec;12(1):1709333.
Link to publication: https://doi.org/10.1080/19420862.2019.1709333

4.    HiDi DNA Polymerase Product Family

Lifestyle-specific S-nitrosylation of protein cysteine thiols regulates Escherichia coli biofilm formation and resistance to oxidative stress.
"... After 3–4 cycles, single clones were isolated and screened by PCR using a high discrimination HiDi polymerase (myPols) and primers with the targeted original or mutated nucleotide at the 3′ end (see Table S5). ..."
Barraud N, Létoffé S, Beloin C, Vinh J, Chiappetta G, Ghigo JM.NPJ Biofilms Microbiomes. 2021 Apr 13;7(1):34.
Link to publication: https://doi.org/10.1038/s41522-021-00203-w

Primordial Germ Cell Migration and Histological and Molecular Characterization of Gonadal Differentiation in Pachón Cavefish Astyanax mexicanus
"...Genotyping was performed using HiDi Taq DNA polymerase (myPOLS Biotec, #9201), which is a highly selective polymerase used for allele-specific PCRs (SNP detection)..."
Imarazene B, Beille S, Jouanno E, Branthone A, Thermes V, Thomas M, Herpin A, Rétaux S, Guiguen Y
Sex Dev. 2021 Mar 10;1-18.
Link to publication: https://doi.org/10.1159/000513378

Emergence of artemisinin-resistant Plasmodium falciparum with kelch13 C580Y mutations on the island of New Guinea.
"…At position 6.97 kb, SNP-specific amplification was performed with HiDi DNA polymerase (myPOLS Biotec, Konstanz, Germany)…"
Miotto O, Sekihara M, Tachibana SI, Yamauchi M, Pearson RD, Amato R, Gonçalves S, Mehra S, Noviyanti R, Marfurt J, Auburn S, Price RN, Mueller I, Ikeda M, Mori T, Hirai M, Tavul L, Hetzel MW, Laman M, Barry AE, Ringwald P, Ohashi J, Hombhanje F, Kwiatkowski DP, Mita T.
PLoS Pathog. 2020 Dec 15;16(12):e1009133.
Link to publication: https://doi.org/10.1371/journal.ppat.1009133

Rapid repair of human disease-specific single-nucleotide variants by One-SHOT genome editing.
"…To identify the HDR-targeted clones by SNMD-PCR, the genomic regions surrounding the target loci were amplified using HiDi DNA polymerase (myPOLS Biotec GmbH, Germany) and the corresponding single-nucleotide marker-specific or allele-specific primer pair…"
Yokouchi Y, Suzuki S, Ohtsuki N, Yamamoto K, Noguchi S, Soejima Y, Goto M, Ishioka K, Nakamura I, Suzuki S, Takenoshita S, Era T.
Sci Rep. 2020 Aug 18;10(1):13927.
Link to publication: https://doi.org/10.1038/s41598-020-70401-7

Development of sake yeast haploid set with diverse brewing properties using sake yeast strain Hiroshima no. 6 exhibiting sexual reproduction.
"...MASA typing was performed using high single-nucleotide discrimination (HiDi) Taq DNA polymerase (myPOLS Biotec, Konstanz, Germany)…"
Yamasaki R, Goshima T, Oba K, Kanai M, Ohdoi R, Hirata D, Akao T.
J Biosci Bioeng. 2020 Jun;129(6):706-714.
Link to publication: https://doi.org/10.1016/j.jbiosc.2020.01.005

Influence of EGFR-activating mutations on sensitivity to tyrosine kinase inhibitors in a KRAS mutant non-small cell lung cancer cell line.
"…To detect knock-in mutations, HiDi DNA Polymerase (myPOLS, Konstanz, Germany, US) was used for PCR…"
Tsukumo Y, Naito M, Suzuki T.
PLoS One. 2020 Mar 4;15(3):e0229712.
Link to publication: https://doi.org/10.1371/journal.pone.0229712

Eliminating primer dimers and improving SNP detection using self-avoiding molecular recognition systems.
"…Indeed, with HiDi DNA polymerase, SNP discrimination is outstanding…"
Yang Z, Le JT, Hutter D, Bradley KM, Overton BR, McLendon C, Benner SA.
Biol Methods Protoc. 2020 Feb 10;5(1):bpaa004.
Link to publication: https://doi.org/10.1093/biomethods/bpaa004
 
Compensation of Disabled Organogeneses in Genetically Modified Pig Fetuses by Blastocyst Complementation.
"…For some chimeric fetuses, allele-specific PCR using Hi-Di DNA polymerase (myPOLS Biotec, Konstanz, Germany) was performed using allele-specific primers, which allowed to amplify the KDR-KO allele (host embryo derived) efficiently…"
Matsunari H, Watanabe M, Hasegawa K, Uchikura A, Nakano K, Umeyama K, Masaki H, Hamanaka S, Yamaguchi T, Nagaya M, Nishinakamura R, Nakauchi H, Nagashima H.
Stem Cell Reports. 2020 Jan 14;14(1):21-33
Link to publication: https://doi.org/10.1016/j.stemcr.2019.11.008

A New Protocol for the Detection of Sterigmatocystin-producing Aspergillus Section Versicolores Using a High Discrimination Polymerase.
"…Since these fungi are found in varied environmental milieu including indoor dust and food products, our aim was to develop a sensitive and convenient assay to detect STC producing fungal strains. We made use of a high discrimination DNA polymerase (HiDi DNA polymerase), for single nucleotide polymorphism (SNP-based PCR amplification)…"
Kubosaki A, Kobayashi N, Watanabe M, Yoshinari T, Takatori K, Kikuchi Y, Hara-Kudo Y, Terajima J, Sugita-Konishi Y.
Biocontrol Sci. 2020 Jan;25(2):113-118
Link to publication: https://doi.org/10.4265/bio.25.113

CRISPR-Cas3 induces broad and unidirectional genome editing in human cells.
"…Single nucleotide substitutions with dsDNA via HDR mediated by CRISPR-Cas3 and -Cas9 were confirmed by HiDi PCR and sequencing in edited 293T cells…"
Morisaka H, Yoshimi K, Okuzaki Y, Gee P, Kunihiro Y, Sonpho E, Xu H, Sasakawa N, Naito Y, Nakada S, Yamamoto T, Sano S, Hotta A, Takeda J, Mashimo T.
Nat Commun. 2019 Dec 6;10(1):5302
Link to publication: https://doi.org/10.1038/s41467-019-13226-x

END-phenomenon negative bovine viral diarrhea virus that induces the host's innate immune response supports propagation of BVDVs with different immunological properties.
"…Because vBVD12− and vBVD12−/P8L differ only by a one-base mutation in the region encoding Npro (at nucleotide 410), it is possible to discriminate the sequences of the respective viruses using HiDi DNA polymerase…"
Shiokawa M, Omatsu T, Katayama Y, Nishine K, Fujimoto Y, Uchiyama S, Kameyama KI, Nagai M, Mizutani T, Sakoda Y, Fukusho A, Aoki H.
Virology. 2019 Dec;538:97-110
Link to publication: https://doi.org/10.1016/j.virol.2019.09.016

Loss of ALBINO3b Insertase Results in Truncated Light-Harvesting Antenna in Diatoms.
"…Both alleles were amplified separately using HiDi polymerase (myPols) according to the manufacturer’s instructions…"
Nymark M, Volpe C, Hafskjold MCG, Kirst H, Serif M, Vadstein O, Bones AM, Melis A, Winge P.
Plant Physiol. 2019 Nov;181(3):1257-1276
Link to publication: https://doi.org/10.1104/pp.19.00868

Lhcx proteins provide photoprotection via thermal dissipation of absorbed light in the diatom Phaeodactylum tricornutum.
"…PCR was performed using HiDi polymerase according to the manufacturer’s instructions for 30 cycles (MyPols, Germany), with either the primer pairs Lhcx1_wild-type-fw/Lhcx1_wild-type-rev and Lhcx1_all-fw/Lhcx1-all_rev or with Lhcx1_comp-fw/Lhcx1_wild-type-rev and Lhcx1_all-fw/Lhcx1-all_rev…"
Buck JM, Sherman J, Bártulos CR, Serif M, Halder M, Henkel J, Falciatore A, Lavaud J, Gorbunov MY, Kroth PG, Falkowski PG, Lepetit B.
Nat Commun. 2019 Sep 13;10(1):4167
Link to publication: https://doi.org/10.1038/s41467-019-12043-6

Bindel-PCR: a novel and convenient method for identifying CRISPR/Cas9-induced biallelic mutants through modified PCR using Thermus aquaticus DNA polymerase.
"…Notably, under the stringent conditions (where Mg2+ concentration was lowered), HiDi DNA polymerase tended to show superior performance relative to rTaq DNA polymerase in terms of the inability to amplify samples harbouring indels…. Furthermore, HiDi DNA polymerase was also superior in terms of the ability to detect the WT samples among samples enriched with mosaic mutations…"
Sakurai T, Kamiyoshi A, Takei N, Watanabe S, Sato M, Shindo T.
Sci Rep. 2019 Jul 9;9(1):9923
Link to publication: https://doi.org/10.1038/s41598-019-46357-8

A strategy to complement PtAUREO1a in TALEN knockout strains of Phaeodactylum tricornutum
S Madhuri, CR Bártulos, M Serif, B Lepetit, PG Kroth
Algal Research, 2019 May, 101469
Link to publication: https://doi.org/10.1016/j.algal.2019.101469

GPR31-dependent dendrite protrusion of intestinal CX3CR1+ cells by bacterial metabolites.
"…To distinguish the difference in the expression between Gpr31b and other Gpr31 isoforms, PCR was performed with HiDi polymerase (myPOLS Biotec), which discriminates primers with a mismatch at the 3′-end…"
Morita N, Umemoto E, Fujita S, Hayashi A, Kikuta J, Kimura I, Haneda T, Imai T, Inoue A, Mimuro H, Maeda Y, Kayama H, Okumura R, Aoki J, Okada N, Kida T, Ishii M, Nabeshima R, Takeda K.
Nature. 2019 Feb;566(7742):110-114
Link to publication: https://doi.org/10.1038/s41586-019-0884-1

Precise and efficient nucleotide substitution near genomic nick via noncanonical homology-directed repair.
"…We further confirmed gene editing by PCR using high single-nucleotide discrimination (HiDi) DNA polymerase that efficiently discriminates primers with a mismatch at the 3′ end … The edited alleles, but not non-edited alleles, can be amplified by HiDi PCR using the edited allele-specific PCR primer…. "
Nakajima K, Zhou Y, Tomita A, Hirade Y, Gurumurthy CB, Nakada S.
Genome Res. 2018 Feb;28(2):223-230
Link to publication: https://doi.org/10.1101/gr.226027.117

One-step generation of multiple gene knock-outs in the diatom Phaeodactylum tricornutum by DNA-free genome editing.
"…The highly selective HiDi DNA polymerase (myPOLS, Germany) was used for allele-specific PCR, together with primers ending with single nucleotide polymorphisms..."
Serif M, Dubois G, Finoux AL, Teste MA, Jallet D, Daboussi F.
Nat Commun. 2018 Sep 25;9(1):3924
Link to publication: https://doi.org/10.1038/s41467-018-06378-9