Volcano Product Family

The Volcano Product family - suitable variants for special requirements

myPOLS Biotec is coming from a scientific tradition: Our expertise lies especially in the development of DNA polymerase assays and in the engineering of DNA polymerases with new properties and functions. The Volcano product family is based on a polymerase engineered by our scientists.

Special products for special requirements
All products of the Volcano family are applicable for reverse transcription reactions and have a thermostable nature. The specialty: Primers should be re-designed for higher melting/annealing points (above 65°C) and additionally, RT-PCRs with temperature gradients are performed to establish the best working PCR protocol for your assay with Volcano products.

Depending on the employed assay, the reverse transcription steps may be omitted (0-step RT-PCR).

0-step RT-PCR

Further development to a new generation of reverse transcriptase - let's do it together
You want to speed up your existing RT-PCR assays or looking for optimized components to develop a new test? Want to have more sensitivity? Let´s do this together. The in-house development facilitates further development. We know our enzymes. This enables us to respond to customer requirements and to adapt the products ideally to specific customer needs and targets.

For example: Some products are already successfully used in IVD products for the pathogenic detection of RNA viruses, like SARS-CoV-2.

 

These are the Volcano “standard” products:

These Volcano components are interesting for you if you want to establish new tests or optimize existing tests with new procedures - for research only use and diagnostic purpose.

RevTaq RT-PCR DNA polymerase: the pure, reverse transcription active enzyme

RevTaq RT-PCR DNA polymerase is an engineered, extremely thermostable reverse transcriptase and combined DNA polymerase, obtained through directed, artificial evolution. (to the shop)

Volcano3G® RT-PCR Master Mixes: different variants for different needs
Volcano3G® RT-PCR Master Mix products contain all components required for general RT-qPCR. The products are offered for research use only and for in-vitro diagnostic purpose.

  • Volcano3G® RT-PCR Probe 2x Master Mix (+high/low ROX) (to the shop)
  • Volcano3G® RT-PCR 2x Master Mix (+GreenDye) (to the shop)
  • Volcano3G® RT-PCR 2x Master Mix (+GreenDye, +high ROX/low ROX) (to the shop)
  • Volcano3G® RT-PCR Probe 2x Master Mix IVD (to the shop)


Direct SARS-CoV-2 RT-PCR detection kit w/o RNA extraction
The kit contains Volcano3G® RT-PCR Probe 2x Master Mix and a duplex primer/probe mix for both SARS-CoV-2 (N1 gene specific) and RNaseP control. Please also refer the procedure as detailed in our recent reference: "Detection of SARS-CoV-2 from raw patient samples by coupled high temperature reverse transcription and amplification" (to the shop)


Find all Volcano products at a glance (to the shop)


Interested? You can contact us for a special quote or more details using our contact form. Products for research only use can be purchased through the shop.