- allele-specific PCR (asPCR), allele-specific amplification (ASA)
- HLA genotyping
- analysis of single CpG methylation sites by PCR (methylation specific PCR, MSP)
- mutation detection by PCR even in a high background of wild-type sequences
- genotyping e.g., in CRISPR/Cas and TALEN approaches
Download: HiDi® DNA Polymerase - Application note
Lifestyle-specific S-nitrosylation of protein cysteine thiols regulates Escherichia coli biofilm formation and resistance to oxidative stress.
Emergence of artemisinin-resistant Plasmodium falciparum with kelch13 C580Y mutations on the island of New Guinea."…At position 6.97 kb, SNP-specific amplification was performed with HiDi DNA polymerase (myPOLS Biotec, Konstanz, Germany)…"
"... After 3–4 cycles, single clones were isolated and screened by PCR using a high discrimination HiDi polymerase (myPols) and primers with the targeted original or mutated nucleotide at the 3′ end (see Table S5). ..."
Barraud N, Létoffé S, Beloin C, Vinh J, Chiappetta G, Ghigo JM.NPJ Biofilms Microbiomes. 2021 Apr 13;7(1):34.
Link to publication: https://doi.org/10.1038/s41522-021-00203-w
Miotto O, Sekihara M, Tachibana SI, Yamauchi M, Pearson RD, Amato R, Gonçalves S, Mehra S, Noviyanti R, Marfurt J, Auburn S, Price RN, Mueller I, Ikeda M, Mori T, Hirai M, Tavul L, Hetzel MW, Laman M, Barry AE, Ringwald P, Ohashi J, Hombhanje F, Kwiatkowski DP, Mita T.
PLoS Pathog. 2020 Dec 15;16(12):e1009133.
Link to publication: https://doi.org/10.1371/journal.ppat.1009133Rapid repair of human disease-specific single-nucleotide variants by One-SHOT genome editing."…To identify the HDR-targeted clones by SNMD-PCR, the genomic regions surrounding the target loci were amplified using HiDi DNA polymerase (myPOLS Biotec GmbH, Germany) and the corresponding single-nucleotide marker-specific or allele-specific primer pair…"
Yokouchi Y, Suzuki S, Ohtsuki N, Yamamoto K, Noguchi S, Soejima Y, Goto M, Ishioka K, Nakamura I, Suzuki S, Takenoshita S, Era T.
Sci Rep. 2020 Aug 18;10(1):13927.
Link to publication: https://doi.org/10.1038/s41598-020-70401-7Development of sake yeast haploid set with diverse brewing properties using sake yeast strain Hiroshima no. 6 exhibiting sexual reproduction."...MASA typing was performed using high single-nucleotide discrimination (HiDi) Taq DNA polymerase (myPOLS Biotec, Konstanz, Germany)…"
Yamasaki R, Goshima T, Oba K, Kanai M, Ohdoi R, Hirata D, Akao T.
J Biosci Bioeng. 2020 Jun;129(6):706-714.
Link to publication: https://doi.org/10.1016/j.jbiosc.2020.01.005 Influence of EGFR-activating mutations on sensitivity to tyrosine kinase inhibitors in a KRAS mutant non-small cell lung cancer cell line."…To detect knock-in mutations, HiDi DNA Polymerase (myPOLS, Konstanz, Germany, US) was used for PCR…"
Tsukumo Y, Naito M, Suzuki T.
PLoS One. 2020 Mar 4;15(3):e0229712.
Link to publication: https://doi.org/10.1371/journal.pone.0229712Eliminating primer dimers and improving SNP detection using self-avoiding molecular recognition systems."…Indeed, with HiDi DNA polymerase, SNP discrimination is outstanding…"
Yang Z, Le JT, Hutter D, Bradley KM, Overton BR, McLendon C, Benner SA.
Biol Methods Protoc. 2020 Feb 10;5(1):bpaa004.
Link to publication: https://doi.org/10.1093/biomethods/bpaa004 Compensation of Disabled Organogeneses in Genetically Modified Pig Fetuses by Blastocyst Complementation."…For some chimeric fetuses, allele-specific PCR using Hi-Di DNA polymerase (myPOLS Biotec, Konstanz, Germany) was performed using allele-specific primers, which allowed to amplify the KDR-KO allele (host embryo derived) efficiently…"
Matsunari H, Watanabe M, Hasegawa K, Uchikura A, Nakano K, Umeyama K, Masaki H, Hamanaka S, Yamaguchi T, Nagaya M, Nishinakamura R, Nakauchi H, Nagashima H.
Stem Cell Reports. 2020 Jan 14;14(1):21-33
Link to publication: https://doi.org/10.1016/j.stemcr.2019.11.008A New Protocol for the Detection of Sterigmatocystin-producing Aspergillus Section Versicolores Using a High Discrimination Polymerase."…Since these fungi are found in varied environmental milieu including indoor dust and food products, our aim was to develop a sensitive and convenient assay to detect STC producing fungal strains. We made use of a high discrimination DNA polymerase (HiDi DNA polymerase), for single nucleotide polymorphism (SNP-based PCR amplification)…"
Kubosaki A, Kobayashi N, Watanabe M, Yoshinari T, Takatori K, Kikuchi Y, Hara-Kudo Y, Terajima J, Sugita-Konishi Y.
Biocontrol Sci. 2020 Jan;25(2):113-118
Link to publication: https://doi.org/10.4265/bio.25.113CRISPR-Cas3 induces broad and unidirectional genome editing in human cells."…Single nucleotide substitutions with dsDNA via HDR mediated by CRISPR-Cas3 and -Cas9 were confirmed by HiDi PCR and sequencing in edited 293T cells…"
Morisaka H, Yoshimi K, Okuzaki Y, Gee P, Kunihiro Y, Sonpho E, Xu H, Sasakawa N, Naito Y, Nakada S, Yamamoto T, Sano S, Hotta A, Takeda J, Mashimo T.
Nat Commun. 2019 Dec 6;10(1):5302
Link to publication: https://doi.org/10.1038/s41467-019-13226-xEND-phenomenon negative bovine viral diarrhea virus that induces the host's innate immune response supports propagation of BVDVs with different immunological properties."…Because vBVD12− and vBVD12−/P8L differ only by a one-base mutation in the region encoding Npro (at nucleotide 410), it is possible to discriminate the sequences of the respective viruses using HiDi DNA polymerase…"
Shiokawa M, Omatsu T, Katayama Y, Nishine K, Fujimoto Y, Uchiyama S, Kameyama KI, Nagai M, Mizutani T, Sakoda Y, Fukusho A, Aoki H.
Virology. 2019 Dec;538:97-110
Link to publication: https://doi.org/10.1016/j.virol.2019.09.016Loss of ALBINO3b Insertase Results in Truncated Light-Harvesting Antenna in Diatoms."…Both alleles were amplified separately using HiDi polymerase (myPols) according to the manufacturer’s instructions…"
Nymark M, Volpe C, Hafskjold MCG, Kirst H, Serif M, Vadstein O, Bones AM, Melis A, Winge P.
Plant Physiol. 2019 Nov;181(3):1257-1276
Link to publication: https://doi.org/10.1104/pp.19.00868Lhcx proteins provide photoprotection via thermal dissipation of absorbed light in the diatom Phaeodactylum tricornutum."…PCR was performed using HiDi polymerase according to the manufacturer’s instructions for 30 cycles (MyPols, Germany), with either the primer pairs Lhcx1_wild-type-fw/Lhcx1_wild-type-rev and Lhcx1_all-fw/Lhcx1-all_rev or with Lhcx1_comp-fw/Lhcx1_wild-type-rev and Lhcx1_all-fw/Lhcx1-all_rev…"
Buck JM, Sherman J, Bártulos CR, Serif M, Halder M, Henkel J, Falciatore A, Lavaud J, Gorbunov MY, Kroth PG, Falkowski PG, Lepetit B.
Nat Commun. 2019 Sep 13;10(1):4167
Link to publication: https://doi.org/10.1038/s41467-019-12043-6Bindel-PCR: a novel and convenient method for identifying CRISPR/Cas9-induced biallelic mutants through modified PCR using Thermus aquaticus DNA polymerase."…Notably, under the stringent conditions (where Mg2+ concentration was lowered), HiDi DNA polymerase tended to show superior performance relative to rTaq DNA polymerase in terms of the inability to amplify samples harbouring indels…. Furthermore, HiDi DNA polymerase was also superior in terms of the ability to detect the WT samples among samples enriched with mosaic mutations…"
Sakurai T, Kamiyoshi A, Takei N, Watanabe S, Sato M, Shindo T.
Sci Rep. 2019 Jul 9;9(1):9923
Link to publication: https://doi.org/10.1038/s41598-019-46357-8A strategy to complement PtAUREO1a in TALEN knockout strains of Phaeodactylum tricornutum
S Madhuri, CR Bártulos, M Serif, B Lepetit, PG Kroth
Algal Research, 2019 May, 101469
Link to publication: https://doi.org/10.1016/j.algal.2019.101469GPR31-dependent dendrite protrusion of intestinal CX3CR1+ cells by bacterial metabolites."…To distinguish the difference in the expression between Gpr31b and other Gpr31 isoforms, PCR was performed with HiDi polymerase (myPOLS Biotec), which discriminates primers with a mismatch at the 3′-end…"
Morita N, Umemoto E, Fujita S, Hayashi A, Kikuta J, Kimura I, Haneda T, Imai T, Inoue A, Mimuro H, Maeda Y, Kayama H, Okumura R, Aoki J, Okada N, Kida T, Ishii M, Nabeshima R, Takeda K.
Nature. 2019 Feb;566(7742):110-114
Link to publication: https://doi.org/10.1038/s41586-019-0884-1Precise and efficient nucleotide substitution near genomic nick via noncanonical homology-directed repair."…We further confirmed gene editing by PCR using high single-nucleotide discrimination (HiDi) DNA polymerase that efficiently discriminates primers with a mismatch at the 3′ end … The edited alleles, but not non-edited alleles, can be amplified by HiDi PCR using the edited allele-specific PCR primer…. "
Nakajima K, Zhou Y, Tomita A, Hirade Y, Gurumurthy CB, Nakada S.
Genome Res. 2018 Feb;28(2):223-230
Link to publication: https://doi.org/10.1101/gr.226027.117One-step generation of multiple gene knock-outs in the diatom Phaeodactylum tricornutum by DNA-free genome editing."…The highly selective HiDi DNA polymerase (myPOLS, Germany) was used for allele-specific PCR, together with primers ending with single nucleotide polymorphisms..."
Serif M, Dubois G, Finoux AL, Teste MA, Jallet D, Daboussi F.
Nat Commun. 2018 Sep 25;9(1):3924
Link to publication: https://doi.org/10.1038/s41467-018-06378-9