The fluorescence of GreenDye is benchmarked using a qPCR assay for the detection of a sequence in genomic HeLa DNA. PCRs were performed with 1 ng/µl of HeLa gDNA in the presence of either GreenDye 1x, EvaGreen® 1x or SYBR® Green I 1x in combination with HiDi DNA polymerase.
The real-time PCR curve with 1x GreenDye (green) in comparison with 1x EvaGreen (grey) and 1x SYBR® Green I (blue) shows the lowest ct-value.
The melting curves are acquired from the PCR experiment described above. All three dyes show a single peak indicating a single amplification product. GreenDye however, shows the sharpest melting peak.