GreenDye 20x

GreenDye 20x

    • Boost your qPCR results without inhibition with GreenDye!

      GreenDye is a highly sensitive DNA binding dye suitable for qPCR applications and melting curve analysis.
      GreenDye shows very low DNA polymerase inhibition when compared with other dyes in PCR. It features a very high fluorescence when bound to double-stranded DNA and exhibits no degradation during PCR cycling. It is a perfect dye for qPCR and high resolution melting applications.

    • - real-time PCR (FAM channel)
      - melt curve analysis
      - High Resolution Melting (HRM)
      - qPCR
      - real-time primer extension
      - screening

      It is recommended to use GreenDye at 1x concentration.
      For example, add 1.25 μl GreenDye to a 25 µl PCR reaction. The fluorescence is measured at the usual SYBR/FAM channels.

    • This product is shipped in cool packs. Please store the product upon arrival at - 20 °C. Minimize the number of freeze-thaw cycles by storing in aliquots. For a day-to-day use, we recommend keeping an aliquot at 4 °C.
    • GreenDye 20x is tested together with HiDi DNA polymerase (#9001) in a real-time PCR. A sequence in genomic HeLa DNA is used as a target to validate ct- and fluorescence values of GreenDye at 1x concentration.
      Please inquire more information at info@mypols.de for lot-specific information.
      No contamination has been detected in standard test reactions.

Benchmarking GreenDye against competitors

The fluorescence of GreenDye is benchmarked using a qPCR assay for the detection of a sequence in genomic HeLa DNA. PCRs were performed with 1 ng/µl of HeLa gDNA in the presence of either GreenDye 1x, EvaGreen® 1x or SYBR® Green I 1x in combination with HiDi DNA polymerase.

The real-time PCR curve with 1x GreenDye (green) in comparison with 1x EvaGreen (grey) and 1x SYBR® Green I (blue) shows the lowest ct-value.

GreenDye Melt Curve Analysis

The melting curves are acquired from the  PCR experiment described above. All three dyes show a single peak indicating a single amplification product. GreenDye however, shows the sharpest melting peak.


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