With Volcano3G RT-PCR master mixes, RT-PCR becomes easier once again. Volcano3G RT-PCR master mixes enable amplification of RNA target sequences with quick and easy PCR protocols, even including 0-step amplification.
No isothermal reverse transcription step is needed: A fast start function due to a hotstart aptamer and the engineered, truly thermostable Taq DNA polymerase with reverse transcriptase activity allows immediate cycling.
Even directRT-PCRs are possible like this: Sample extraction and sample lysis is not necessary as immediate hot PCR cycling conditions can brake cell- and virus membranes. Please see our published references to this product.