Direct SARS-CoV-2 RT-PCR detection kit w/o RNA extraction

Acceleration of SARS-CoV-2 testing: Simply skip the step of RNA extraction.

The kit contains Volcano3G® RT-PCR Probe 2x Master Mix and a duplex primer/probe mix for both SARS-CoV-2 (N1 gene specific) and RNaseP control.

Download the manual here (Updated)

To learn more about the use of SARS-CoV-2 RT-PCR detection kit w/o RNA extraction, please take a look at the procedure detailed in our recent reference: "Detection of SARS-CoV-2 from raw patient samples by coupled high temperature reverse transcription and amplification"

This publication shows:

“The approach delivers results without the need to purify RNA, reduces handling steps, minimizes costs, and allows evaluation by non-specialized equipment. The use of unprocessed swap samples is enabled by employing a heat-stable RNA- and DNA-dependent DNA polymerase, which performs the double task of stringent reverse transcription of RNA at elevated temperatures as well as PCR amplification of a SARS-CoV-2 specific target gene .” (Quote from the publication)

The University of Konstanz is currently using an adapted procedure employing the Direct SARS-CoV-2 RT-PCR detection kit w/o RNA extraction in a campus-wide surveillance study.

Please note: For research use only. For clinical testing, you may use this assay as a lab developed test validated by you following the applicable regulatory requirements.


Collections: RNA / RT-PCR



Faster

No isothermal reverse transcription step is needed:  A fast start function due to a hotstart aptamer and the  engineered, truly thermostable Taq DNA polymerase with reverse transcriptase activity allows immediate cycling. 

Even directRT-PCRs are possible like this: Sample extraction and sample lysis is not necessary as immediate hot PCR cycling conditions can brake cell- and virus membranes. Please see our published references to this product.


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