Skip the reverse transcription step. Isothermal reverse-transcription steps are not needed.
Volcano2G is a PCR-active reverse transcriptase and extremely thermostable with a half-life at 95°C of >40 min.
Volcano2G DNA polymerase can also be used for real-time PCR, when adding a real-time PCR dye. Volcano2G has a Taq DNA polymerase-like nuclease domain making it also suitable for hydrolysis probe based assays. As demonstrated here, NONO mRNA was detected using a 10-fold dilution series of total RNA by using a probe-based qPCR assay and the zero-step RT-PCR protocol.
For a reliable RT-PCR of cellular RNAs, sample preparation steps are indispensable. However, these steps are laborious, time consuming and necessitate extra consumables. With our VolcanoCell2G RT-PCR 2x Master Mix you can substitute the laborious RNA preparation with a fast and convenient 'zero-step' protocol.
VolcanoCell2G RT-PCR 2x Master mix has a streamlined 'zero-step' workflow that allows you to circumvent laborious purification and extraction steps, saving you both time and money.
VolcanoCell2G RT-PCR 2x achieves detection sensitivities down to single cells. In this example, GAPDH mRNA was detected from a single Lund human mesencephalic (LUHMES) cell following the VolcanoCell2G protocol.
A publication with more examples can be found in the Publications section.