€349,00
Isotherm2G is a mesophilic DNA polymerase, perfectly suited for Isothermal amplifcations. It synthesizes DNA from both DNA and RNA templates with a high strand displacement activity. Isotherm2G has been mutated in order to have improved reverse transcription activity. It is most active between 55-70°C. Isotherm2G DNA polymerase is the choice No. 1 for isothermal amplification reactions such as loop-mediated isothermal amplification (LAMP), strand displacement amplification (SDA), rolling circle amplification (RCA), ramification amplification (RAM), genome amplification and more.
Isotherm2G DNA polymerase is supplied as a 8 U/µl solution containing glycerol. It comes together with a sufficient amount of 10x reaction buffer which has been optimized for isothermal amplifications. The buffer contains 3.25 mM Magnesium-ions in the final 1x concentration. The optimal Magnesium, Primer and dNTPs concentration strongly depend on the applied application and should be optimized.
This product is shipped on cool packs. It is recommended to store the product upon arrival at -20°C.
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