Isotherm2G DNA polymerase

Isotherm2G DNA polymerase

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• Isotherm2G is a mesophilic DNA polymerase, perfectly suited for Isothermal amplifcations. It synthesizes DNA from both DNA and RNA templates with a high strand displacement activity. Isotherm2G has been mutated in order to have improved reverse transcription activity. It is most active between 55-70°C. Isotherm2G DNA polymerase is the choice No. 1 for isothermal amplification reactions such as loop-mediated isothermal amplification (LAMP), strand displacement amplification (SDA), rolling circle amplification (RCA), ramification amplification (RAM), genome amplification and more.

• Isotherm2G DNA polymerase is supplied as a 8 U/µl solution containing glycerol. It comes together with a sufficient amount of 10x reaction buffer which has been optimized for isothermal amplifications. The buffer contains 3.25 mM Magnesium-ions in the final 1x concentration. The optimal Magnesium, Primer and dNTPs concentration strongly depend on the applied application and should be optimized.

• This product is shipped on cool packs. It is recommended to store the product upon arrival at -20°C.

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