qPCR Probe LyoBeads
are ready-to-use freeze-dried master mixes that¬†contain all components necessary for a rapid, sensitive and reproducible detection and quantification of DNA and cDNA. qPCR Probe LyoBeads are¬†shipped and stored simply at room-temperature in a dry place. This provides¬†a more cost efficient and ecological distribution compared¬†to¬†other master mixes. All necessary components are already included in one¬†LyoBead: an engineered DNA polymerase, an optimized reaction buffer and ultrapure dNTPs. Only primers and probes need to be added.¬†A hot-start formulation of the included DNA polymerase prevents false amplification during the reaction set-up.
|#2201S||qPCR Probe LyoBeads pre-dispensed||1 x 96 well-plate||¬†‚ā¨ 100,-|
|#2201M||qPCR Probe LyoBeads pre-dispensed||5 x 96 well-plate||¬†‚ā¨ 450,-|
|#2201C||customized qPCR Probe LyoBeads already containing target specific primers&probes||-||Please inquire|
Bulk offer: Should you require larger amounts (>1500 beads), significant discounts are available. Customized qPCR Probe LyoBead production and pre-distribution will be provided on demand as well (further information on possible pre-distributing formates please read the¬†detailed product description¬†below). Please contact us to receive an individual quotation.
Specific quantification over a broad dynamic range.
TaqMan-Assay for parvovirus B19, triple determination on a respective plasmid dilution series. Tests were carried out on 109-1 copies/rxn.
|LyoBead||0.75 ¬Ķl¬†(volume of one LyoBead)|
|Primers & Probes||x ¬Ķl|
|Water||fill up to 20 ¬Ķl total reaction volume|
For non-dispensed LyoBeads, aliquote them with a metallic tweezer into individual reaction tubes or wells as it is shown in this short video.
For already pre-dispensed Lyobeads, briefly tap-down or gently spin down before opening the reaction tubes.
Add primers and probes as well as template/sample extract. Close the PCR tubes, vortex or mix briefly, spin down and start the PCR.
- Multiplex PCR
- Realtime PCR
- Probe-based PCR
- Pathogene detection
- qPCRs (Probe-based)
- Primer extensions¬†
- Endpoint PCRs
- Screening / High-throughput PCRs
Typical PCR protocol
Typical PCR protocol
|Initial denaturation||95¬įC||2 min|
|Annealing*||54-72¬įC||30 sec¬† ¬† ¬†(25-40 cycles)|
|Extension||72¬†¬įC||1 min / 1000 bp|
*Typically, the annealing temperature is about 3-5¬įC below the calculated melting temperature of the primers used.¬†
Detailed product description
Pre-dispensed qPCR Probe LyoBeads
Each qPCR Probe LyoBead is equivalent to a 20 ¬Ķl PCR reaction and can either be provided pre-dispensed on demand or non-dispensed. qPCR Probe LyoBeads can be pre-dispensed in¬† 8- or 12-strip PCR tubes (upper image), single PCR tubes or in 96-well plates (as you can see in this video here). Non-dispensed qPCR Probe LyoBeads are provided in micro tubes with screw cap (as shown in the lower image).
Handling of non-dispensed qPCR Probe LyoBeads
It is recommended to use a metallic tweezer to aliquote the qPCR Probe LyoBeads as shown¬†in this short video.
qPCR Probe LyoBeads are tested in real-time qPCR. The product demonstrates linearity of amplification over a specified serial dilution of human genomic DNA. DNA polymerase activity: DNA polymerase activity has been monitored and adjusted to a specific DNA polymerase activity using an artificial DNA template and a DNA primer. Enzyme-concentration has been determined by proteinspecific staining. Please inquire more information at firstname.lastname@example.org for the lot-specific concentration. No contamination has been detected in standard test reactions.
Costly cooling chains and storage at -20¬įC are no longer necessary: ¬†qPCR Probe LyoBeads are¬†shipped and stored simply at room-temperature in a dry place. Once opened, the surrounding air humidity is ideally less than 30%. ¬†
Composition / Content of the Product
qPCR Probe LyoBeads¬†contain all the components necessary for PCR, including a hot-start formulated¬†Taq DNA polymerase and an optimized buffer including ultrapure dNTPs.
Which DNA polymerase¬†is included in these LyoBeads?
The LyoBeads contain our best performing, hot-start formulated, engineered Taq DNA polymerase. We have chosen this DNA polymerase as it is best suited for multiplex PCRs, pathogen detection qPCRs and all kinds of probe-based qPCRs.
The enzyme's fidelity is comparable with the wild-type Taq DNA polymerase as described in the reference below.¬†
Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase.
Biochemistry 1988; 27(16): 6008‚Äď6013. K. R. Tindall, T. A. Kunkel.
How can I avoid electro static charge of the LyoBeads?
We recommend to use metallic tweezers for distributing the LyoBeads into the reaction-tubes. Also anti-static equipment and gloves may prevent electro static problems. It also helps to stand the PCR tubes or plates on a metallic surface.
Can you also produce LyobBeads containing other DNA polymerases?
Yes we can. Currently we are working on qPCR LyoBeads containing GreenDye and RT-qPCR Beads containing a Volcano2G mutant for you. On request we can also discuss the production of other LyoBeads with you. Please contact us should you be interested!
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